Trevigen 8-oxo-dG

HT 8-oxo-dG ELISA Kit

8-hydroxy-2’-deoxyguanosine (8-oxo-dG) is a modified nucleoside, which is the most commonly studied and detected by-product of DNA damage that is excreted following DNA repair. The presence of 8-oxo-dG and its analogs, 8-hydroxyguanosine and 8-hydroxyguanine in the urine, is associated with many degenerative disease states in which reactive oxygen species (ROS) contribute to pathogenesis. 8-oxo-dG, a biomarker of oxidative stress, has been employed as an ROS indicator in many studies on a wide variety of diseases.

Trevigen’s 8-oxo-dG ELISA (enzyme-linked immunosorbent assay) is a fast and sensitive, competitive immunoassay for the detection and quantitation of 8-hydroxy-2'-deoxyguanosine (8-oxo-dG) in saliva, urine and serum samples. This kit features a 96 strip well plate pre-bound with 8-oxo-dG, an 8-oxo-dG monoclonal antibody, an enzyme-labeled secondary antibody, detection substrates and buffer to provide a complete, robust assay flexible for your experimental design.

FEATURES:

• Colorimetric format
• High throughput 96 test size
• Standard curve has a range of 0.94 - 60 ng/ml
• Sensitive to 0.59 ng/ml

APPLICATIONS :

For the detection and quantitation of 8-hydroxy-2'-deoxyguanosine (8-oxo-dG) in urine, serum and saliva samples.

STORAGE:

All reagents are stable as supplied at 4°C, except the 8-oxo-dG standard, which should be stored at -20°C. For optimum storage, the 8-oxo-dG standard should be aliquoted into smaller portions and stored at -20°C to avoid repeated freeze/ thaw cycles. Unused wells of the 8-oxo-dG Immunoassay Plate should be resealed with desiccant in the foil pouch provided and stored at 4°C until the kit expiration date.

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